1. SCENE ASSESSMENT AND AREA CONTROL: Verify the spill source is controlled and all injured parties have received care and been removed from the area. Secure the perimeter to prevent unauthorized personnel from entering the contaminated zone, and visually confirm the spill volume is within the kit's manageable range before proceeding.

2. DON PPE BEFORE ANY CONTACT: Open the kit on a clean surface located outside the spill zone. Don the gown first and tie it securely, then don the eye shield, then don the medical-grade gloves, pulling the cuffs over the gown sleeves to maintain barrier integrity.

3. APPLY FLUID-SOLIDIFYING POWDER: Open the fluid-solidifying powder packet carefully and sprinkle it evenly over the spill, working from the outer edge inward. Avoid dumping the powder from height to prevent aerosolization. Allow 1 to 2 minutes for full absorption into a gel-like consistency before proceeding.

4. COLLECT AND BAG SOLIDIFIED WASTE: Open and position the red biohazard bag near the spill area but not within it. Use the bio-scoop and detachable scraper to transfer all solidified material into the red bag. Wipe residual contamination using paper towels in an outside-in circular pattern, then bag the towels. Place the used bio-scoop and scraper into the red biohazard bag.

5. SEAL RED BAG AND DOFF PPE: With gloves still on, seal the red biohazard bag and place it inside the secondary black or white trash bag. Remove gloves inside-out and dispose of them in the trash bag. Remove the gown inside-out and dispose of it in the trash bag. Remove the eye shield by the clean earpieces, dispose of it in the trash bag, and seal the trash bag.

6. DECONTAMINATE HANDS AND REPORT: Use the BZK antiseptic towelette to clean hands immediately following PPE removal. Follow with thorough handwashing using soap and water for a minimum of 20 seconds. Dispose of all sealed waste per facility policy and applicable biohazard regulations, and report the incident and kit use to the appropriate supervisor.